Reporter

Part:BBa_K1891012:Design

Designed by: Zhao Jingyu   Group: iGEM16_BNU-China   (2016-10-10)


YCE+beta-tubulin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 542
    Illegal BamHI site found at 1325
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 583
    Illegal AgeI site found at 391
    Illegal AgeI site found at 721
    Illegal AgeI site found at 1489
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We connected this two gene fragments using fusion PCR. First we acquired YCE gene fragment and beta-tubulin gene fragment. And then we redesigned a antisense primer of YCE and a sense primer of beta-tubulin with a linker about 42bp. And then doing fusion PCR.


Source

Beta-tubulin comes from human breast cancer cell line Mcf-7. YCE comes from pSPYCE plasmid.

References